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Progressive Stochastic Reconstruction Technique for Cryo Electron Tomography

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Abstract:

Cryo Electron Tomography (cryoET) plays an essential role in Structural Biology, as it is the only technique that allows to study the structure and intracellular distribution of large macromolecular complexes in their (close to) native environment. A major limitation of cryoET is the highest achievable resolution, currently at around 3 nm, which prevents its application to smaller complexes and in turn to a wider range of important biological questions.